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Melanoma metastasis with a high contiguous infiltration of ECP expressing eosinophils and CD8 expressing effector T-cells. Paraffin-embedded tissue samples of a metastasis are co-stained with immunofluorescent anti-ECP antibodies (marked green) and anti-CD8 antibodies (marked red). Nuclei were stained in blue with DAPI. Abbreviations: ECP: eosinophil cationic protein; CD: cluster of differentiation; DAPI = 4′,6-Diamidin-2-phenylindol.
