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Current ratio by the concentration of fluorescent reagent. (a) AF488-λem: 490 nm; (b) AF488-λem: 590 nm; (c) AF488-λem: 680 nm; (d) AF594-λem: 490 nm; (e) AF594-λem: 590 nm; (f) AF594-λem: 680 nm; (g) AF680-λem: 490 nm; (h) AF680-λem: 590 nm; (i) AF680-λem: 680 nm.
