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Ubiquitylation participates in the degradation of AMT1; 3 proteins under high ammonium stress. (A) Total proteins were isolated from AMT1;3-EGFP seedlings that had been treated with DMSO and MG132, in the presence of CHX, with high ammonium treatment for 0, 15, 30, 60 min. The total proteins were separated by SDS-PAGE and immune detected with GFP antibody. (B) Relative contents of AMT1;3-EGFP (per unit of total protein). Error bars represent standard errors.
