Figure 5.

Effects of APOE genotype on Aβ level in primary neurons. Primary cortical neurons cultures were obtained from P0-P1 newborn KI mice carrying the human APOE3 or APOE4 alleles. After 5, 9 or 12 days in culture (DIV5, DIV9 and DIV12), levels of Aβ38, Aβ40 and Aβ42 were assessed in the culture medium using the MSD multiplex ELISA (pg/mg of proteins measured in cells lysates). Statistics: two-way ANOVA mean +/− SEM; **: p < 0.01, and ****: p < 0.0001, comparison with APOE3, 3 independent cultures with 2 to 3 replicates per culture.