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. 2022 Nov 19;14(22):5687. doi: 10.3390/cancers14225687

Figure 5.

Figure 5

Lin28 inhibitors block the Lin28A isoform. (A) EMSA used 10 nM of IR800DyeCWN-labeled Let-7 probe (lane 1), 0.5 uM Lin28A ZKD protein with 10 nM of labeled Let-7 probe (lane 2), or Lin28A ZKD, labeled Let-7 probe and 100× non-labeled competitor probe (lane 3). EMSA used Lin28A ZKD, labeled Let-7 probe, and 100–1200 uM of indicated compounds (lanes 4–10). (B) FP assays were performed by using 10 uM of Lin28A ZKD, 1 nM of FAM-labeled Let-7 probe, and 1 nM–200 uM of compounds. (C) Lin28A-positive IGROV1 cells were treated with 20 uM of the indicated compound for 48 h. Real-time qPCR measured RNA levels of Let-7d, SOX2, and HMGA2. Three biological replicates were performed and all results are presented as mean +/− SD. p-value ≤ 0.05—(*); ≤0.01—(**); ≤0.001—(***).