Figure 6.

UL43 protein binding to ARL2 and phosphorylation of ANT1. (A) Co--immunoprecipitation assay in mitochondrial proteins of UL43--WT, UL43--Y144A, Mock, performed with co-transfect of ARL2-siRNA. (B) The peptide sequence is detected by mass spectrometry, and the ARL2-specific binding sequence is marked in red. (C) Co-immunoprecipitation assay in mitochondrial proteins of UL43-WT and Mock, detecting the phosphorylation level of BART and ANT1. (D) Fluorescent image of mitochondrial membrane pores opening test, UL43-WT and UL43-Y144A were treated with CoCl₂, ionomycin and ARL2-siRNA. Scale bar = 5 μm. The data shown in each panel are representative of three independent experiments.