TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype | Reference or source |
|---|---|---|
| E. coli strains | ||
| 042 | Wild-type EAEC strain from Peru | 24 |
| DH5α λpir | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 deoR Δ(lacZYA-argF)U169 λpir+ | 8 |
| S17-1 λpir | pro res mod+ RP4-2 Tet::Mu-Kan::Tn7 | 32 |
| HB101 | K-12/B hybrid | 2 |
| JIF1 | 042 pet::pJIF | This study |
| JIF2 | JIF1 harboring pCEFN1 | This study |
| Plasmids | ||
| pJP5603 | 3.1-kb R6K-based suicide vector (Kanr) | 30 |
| pJIF | 1,120-bp internal fragment of pet cloned into pJP5603 (Kanr) | This study |
| pCEFN1 | 3.9-kb PCR-derived fragment encoding Pet protein, cloned into pSPORT1 (Ampr) | 9 |