TABLE 2.
Reactivitya of MAbs with H. pylori strains in relation to α3-fucosyltransferase gene C-tract length
| Strainb | Specificity of MAb used
|
Fucosyltransferase gened
|
||||||
|---|---|---|---|---|---|---|---|---|
| Mono-Lex (6H3)c | Poly-Lex (54.1F6A)c | i antigen | H type 1 | H type 2 | Ley | futB | futA | |
| NCTC 11637 | 0.2 | 2.5 | 0.2 | 2.5 | 0.8 | 1.1 | C9 (off) R8 | C10 (on) R2 |
| K5.1 | 0.2 | 1.6 | 0.1 | 1.6 | NDe | 0.5 | C9 (off) R8 | C10 (on) R2 |
| 2b | 0.4 | 2.5 | 0.2 | 2.3 | ND | 0.9 | C9 (off) R8 | C10 (on) R2 |
| K4.1 | 0.2 | 0.2 | 2.5 | 2.5 | 0.4 | 0.4 | C9 (off) R8 | C11 (off) R2 |
| 1c | 0.4 | 2.5 | 0.1 | 0.9 | 0.7 | 2.5 | C10 (on) R8 | C10 (on) R2 |
| 4187E | 2.5 | 2.5f | 0.1 | 1.3 | 0.1 | 2.5g | C10 (on) R7 | C10 (on) R2 |
| 4187E-KO651 | 0.5 | 2.5f | 0.3 | 2.5 | 0.1 | 1.1 | ND | ND |
| 4187E-KO379 | 2.5 | 2.5f | 1.0 | 1.0 | 0.1 | 2.5g | ND | ND |
| 4187E-KO379/651 | 0.1 | 0.1 | 2.5 | 2.5 | 0.1 | 0.5 | ND | ND |
a
Optical density at 492 nm in ELISA.
b
Strains K5.1, 2b, K4.1, and 1c are LPS-phase variants of strain NCTC 11637.
c
MAbs 6H3 and 54.1F6A recognize monomeric and polymeric Lex, respectively.
d
futB and futA designate the orthologs of H. pylori 26695 α3-fucosyltransferase genes HP0651 and HP0379, respectively. C, C-tract length; R, number of terminal repeats.
e
ND, not done.
f
In titration, this MAb reacted 128-fold more with 4187E-KO651 than with strain 4187E and 64-fold less with 4187E-KO379 than with the 4187E parent.
g
In titration, MAb Hp 151 (anti-Ley) reacted equally well with strains 4187E and 4187E-KO379.