Table 3.
Experimental designs and the results of steepest ascent for 3-Met production.
| Groups | Shaking Speed (rpm) | Temperature (°C) | Glucose Concentration (g/L) | Initial pH | Loading Volume (mL/250 mL) | Inoculum Size (%, v/v) | Time Point of L-Met Addition (h) | Time (h) | Yeast Concentration (g/L) | L-Met Concentraion (g/L) | Tween-80 Concentration (g/L) | 3-Met Concentration (g/L) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 90 | 36 | 20 | 4 | 100 | 0.1 | 30 | 96 | 2.4 | 2 | 0.5 | 0.85 |
| 2 | 120 | 34 | 25 | 4.5 | 87.5 | 0.2 | 24 | 84 | 2 | 3 | 1 | 1.17 |
| 3 | 150 | 32 | 30 | 5 | 75 | 0.3 | 18 | 72 | 1.6 | 4 | 1.5 | 1.84 |
| 4 | 180 | 30 | 35 | 5.5 | 62.5 | 0.4 | 12 | 60 | 1.2 | 5 | 2 | 2.68 |
| 5 | 210 | 28 | 40 | 6 | 50 | 0.5 | 6 | 48 | 0.8 | 6 | 2.5 | 2.87 |
| 6 | 240 | 26 | 45 | 6.5 | 37.5 | 0.6 | 0 | 36 | 0.4 | 7 | 3 | 0.87 |