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. 2022 Nov 3;44(11):5427–5439. doi: 10.3390/cimb44110368

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The analytical specificity tests for detection of PEDV spike gene and PCV2 ORF gene using (A) duplex PCR-based AGE, (B) duplex LAMP-based AGE and (C) duplex LAMP-LFD. Lane M represents 100 bp DNA ladder marker; Lane 1 represents duplex PEDV/PCV2; Lane 2 represents PCV2; Lane 3 represents PEDV; Lanes 4–10 represent Aujeszky’s disease virus (ADV), foot and mouth disease virus (FMDV), transmissible gastroenteritis virus (TGEV), porcine reproductive and respiratory syndrome virus European strain (PRRSV-EU), and porcine reproductive and respiratory syndrome virus US strain (PRRSV-US), respectively.