FIG. 2.

LcrG facilitates targeting of Yops into infected HeLa cells. Y. pestis KIM8-3002 (parent) or Y. pestis KIM8-3002.7 (ΔlcrG3) possessing pBAD18-Kan, pAraG18K (ΔlcrG3 pG), or pAraV18K (ΔlcrG3 pV) was used to infect HeLa cell monolayers at an MOI of 10. Expression of LcrG was induced by addition of arabinose to 2% (wt/vol), and LcrV was induced by addition of IPTG to 0.1 mM. Cell morphology was visualized by phase-contrast microscopy and recorded at 3.5 or 6 h after infection by photography through a green filter.