FIG. 3.

Schematic representation of LcrV proteins. Portions of lcrV were subcloned into expression vectors possessing IPTG-inducible promoters. HT-V (full-length LcrV) and HT-VN68 (N-terminally truncated LcrV) possesses an N-terminal leader sequence containing six His residues. Fusion of the YopE mRNA secretion signal to LcrV in pE15VN68 resulted in the addition of amino acids 1 to 15 of YopE to N-terminally truncated LcrV, yielding the protein E15VN68. It was expressed from the yopE promoter. VC216 represents a C-terminal truncation and lacks the last 111 residues of LcrV.