In vivo PTT assisted by the “parasitism” of ACG hydrogel in the tumor. (A) Infrared thermal photos of tumor-bearing mice during 808 nm laser irradiation, n = 5. (B) The average temperature change curves of mice’s tumor surface during 808 nm laser irradiation, shown as means ± SD, complied with the normal distribution and evaluated by one-way ANOVA tests, ** p < 0.01, *** p < 0.001. (C) The average relative tumor volume (V/V0) change of mice in each group, shown as means ± SD, complied with the normal distribution and evaluated by one-way ANOVA tests, * p < 0.05, ** p < 0.01. (D) The digital photographs of tumor-bearing mice at different time points (Origin, 0, 3, 9, and 15 d) in each group, n = 5. (E) The ratio of tumor weight to body weight of mice in each group, shown as means ± SD, not complied with the normal distribution and evaluated by nonparametric Kruskal–Wallis tests, * p < 0.05, ** p < 0.01. (F) Photo of tumor masses excised from tumor-bearing mice after different treatments. ACG: intratumoral injection of 50 μL ACG hydrogel. L: 808 nm laser irradiation (2 W/cm2, 10 min). ACG (0 h) + L: intratumoral injection of 50 μL ACG hydrogel followed by immediate laser irradiation (2 W/cm2, 10 min). ACG (12 h) + L: intratumoral injection of 50 μL ACG hydrogel followed by 12 h delayed laser irradiation (2 W/cm2, 10 min). TW: Tumor Weight. BW: Body Weight.