FIG. 2.

High-frequency pressure blasts acutely changes neural activity of human cerebral organoids. (A) Representative traces of single-unit events. In blue (top) the average trace of all events from an organoid exposed to 5000 Hz (High), 250 kPa amplitude pressure wave. In orange (bottom) the average trace from the same organoid at 24 h after exposure. (B) Quantification of single unit events normalized to no blast control groups of 1 h (blue) and 24 h (orange) after exposure. (C) Quantification of single-unit even amplitudes. (D) Representative average trace of population spike event from an organoid at 1 h (blue, top) and 24 h (orange, bottom) after exposure. (E) Quantification of population spike event frequency, normalized to control groups. Chi-squared = 15.87, df = 7. (F) Quantification of population event amplitude. Error bars presented as standard error of the mean. *p < 0.05, n = 12 organoids per group. All statistics calculated using one-way analysis of variance except panel (E), which uses chi-squared test. Color image is available online.