TABLE 2.
Effects of DEM on hepatic iNOS expression induced by recombinant TNF-α
| Treatmenta | No. of positive Kupffer cells/HPFb | No. of positive hepatocytes/HPFb | Intensity of positive endothelial cellsbc |
|---|---|---|---|
| Vehicle | 1 ± 1.3 | 64.0 ± 7.3 | + |
| DEM | 0.8 ± 1.3 | 2.5 ± 1.9d | − |
a
Two groups of mice (n = 4/group) received either sesame oil or 5.3 mmol of DEM/kg i.p. 2 h prior to being challenged i.p. with 10 μg of recombinant mouse TNF-α/kg. Twelve hours later, tissues were recovered and iNOS expression was determined.
b
At least five randomly selected HPFs (magnification, ×200) were scored for each tissue section.
c
Intensity scores for endothelial cells were assigned on a scale of − to +++ (see Materials and Methods).
d
The results for this group differed significantly from those for its control (vehicle-treated) group (P < 0.01) as calculated by Student’s t test.