FIG. 2.

Cross-reactivity of MAb B5 with selected immunotypes and mutants of N. meningitidis LPS and O-deacylated (odA) LPS as determined by solid-phase ELISA. LPS glycoforms of immunotypes L2 (35E) (solid bars), L3 (H44/76) (open bars), L4 (89I) (hatched bars), L5 (M981) (open bars), L8 (M978) (horizontal-line-filled bars), wild-type, and respective mutants (galE, lgtA, or lgtB), in a native or O-deacylated form, were coated onto ELISA plates (see Materials and Methods), and the reactivity of MAb B5 was determined by standard ELISA (OD, A₄₁₀).