FIG. 4.

Cross-reactivity of MAb B5 with genetically modified L3 LPS and chemically modified L8 LPS from N. meningitidis, as determined by solid-phase ELISA. LPS glycoforms of immunotype L8 (M978) (horizontal-line-filled bars) chemically modified by O deacylation and HF treatment and immunotype L3 (H44/76) (open bars) galE, icsB, icsA, lsi, and PB4 mutants (O deacylated) were coated onto ELISA plates (see Materials and Methods), and the reactivity of MAb B5 was determined by standard ELISA (OD, A₄₁₀).