FIG. 6.

Silver-stained tricine gels of LPS preparations (10 μg/lane) from N. meningitidis group B strains which were not reactive with MAb B5. These LPS preparations were either not treated (−) or treated with neuraminidase (+) to show the presence of sialic acid. (a) MAb B5-negative strains: lanes 1 and 2, NGE30; lanes 3 and 4, BZ157; lanes 5 and 6, EG328; lanes 7 and 8, 1000; lanes 9 and 10, 3906. (b) MAb B5-negative strains: lanes 1 and 2, EG327; lanes 3 and 4, NGH38; lanes 5 and 6, NGH15; and MAb B5-positive strain: lanes 7 and 8, MC58. The presence of sialic acid is indicated (NeuAc). This band was seen in untreated (−) and removed in treated (+) neuraminidase preparations.