TABLE 4.

Negative-ion ES-MS data and proposed compositions of O-deacylated LPS from galE capsule-deficient mutant N. meningitidis MAb B5-negative strains^a

Strain	Observed ions (m/z)		Molecular mass (Da)			Proposed composition
	(M-2H)2−	(M-H)−	Observed	Calculated	Lipid Ab
1000	1,213.0	2,427.6	2,427.7	2,427.2	1,075	2Glc, GlcNAc, 2Hep, 2 Kdo, Lipid A
	1,252.9	2,507.8	2,507.8	2,507.2	1,155	2Glc, GlcNAc, 2Hep, 2 Kdo, Lipid A
	1,314.5	2,630.9	2,630.9	2,630.3	1,278	2Glc, GlcNAc, 2Hep, 2 Kdo, Lipid A
NGH38	1,293.8	2,589.5	2,589.3	2,589.3	952	3Glc, GlcNAc, 2Hep, PEtn, 2Kdo, Lipid A
EG327	1,151.2	2,304.4	2,304.4	2,304.1	952	2Glc, GlcNAc, 2Hep, 2 Kdo, Lipid A
NGE30	1,132.1			2,265.1	1,075	Glc, GlcNAc, 2Hep, 2 Kdo, Lipid A
	1,396.1	2,793.4	2,793.7	2,792.5	1,075	3Glc, 2GlcNAc, 2Hep, 2 Kdo, Lipid A
	1,436.0	2,873.7	2,873.9	2,872.5	1,155	3Glc, 2GlcNAc, 2Hep, 2 Kdo, Lipid A
	1,498.0	2,997.2	2,997.1	2,995.6	1,278	3Glc, 2GlcNAc, 2Hep, 2 Kdo, Lipid A
BZ157	1,274.6	2,551.4		2,550.3	1,075	2Glc, GlcNAc, 2Hep, PEtn, 2Kdo, Lipid A
	1,314.8	2,631.1	2,631.2	2,630.3	1,155	2Glc, GlcNAc, 2Hep, PEtn, 2Kdo, Lipid A
	1,376.4	2,754.4	2,754.5	2,753.4	1,278	2Glc, GlcNAc, 2Hep, PEtn, 2Kdo, Lipid A
	1,457.5	2,916.6	2,916.6	2,915.6	1,278	3Glc, GlcNAc, 2Hep, PEtn, 2Kdo, Lipid A

^aAverage mass units were used for calculation of molecular weight based on proposed composition as follows: Glc, 162.15; Hep, 192.17; GlcNAc, 203.19; Kdo, 220.18; PEtn, 123.05. Hep, heptose; Kdo, 3-deoxy-d-manno-octulosonic acid; Glc, glucose; GlcNAc, N-acetylglucosamine; PEtn, phosphoethanolamine. 

^bAs determined by MS-MS analyses.