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Loss of biofilm formation in S. aureus ATCC 35556Δica::tet. The ica locus in S. aureus ATCC 35556 was deleted and replaced with a tetracycline resistance cassette by homologous recombination. The knockout strain, ATCC 35556Δica::tet, is unable to form a biofilm in vitro; however, the ability to form a biofilm is restored when the knockout strain is complemented with pSC18, carrying the wild type ica genes. The assay for each strain is shown in duplicate.
