Table 5.
Colonization-related genes.
| Genes | Identity (%) | Genome Position in B. Velezensis B.BV10 (bp) | Described Function According to Subtwiki [30] | |
|---|---|---|---|---|
| abrB | 91.379 | 45,889 | 45,600 | Transcriptional regulator of transition-state genes. |
| comA | 80.380 | 2,996,026 | 2,995,395 | Regulation of genetic competence and quorum sensing. |
| degQ | 86.765 | 2,999,820 | 2,999,685 | Stimulates production of degradative enzymes and extracellular poly-gamma-glutamate; stimulates phosphorylation of DegU by DegS. |
| degU | 86.377 | 3,384,604 | 3,383,915 | Two-component response regulator; regulation of degradative enzyme expression, genetic competence, biofilm formation, and capsule biosynthesis (together with SwrA); non-phosphorylated DegU is required for swarming motility. |
| epsA | 99.294 | 3,289,519 | 3,288,812 | Extracellular polysaccharide synthesis; putative transmembrane modulator of EpsB activity; might activate EpsB autophosphorylation and substrate phosphorylation. |
| epsB | 99.853 | 3,288,806 | 3,288,126 | Extracellular polysaccharide synthesis; protein tyrosine kinase; phosphorylation of EpsE. |
| epsC | 98.941 | 3,287,880 | 3,286,087 | UDP-N-acetylglucosamine 4,6-dehydratase; required for extracellular polysaccharide synthesis; this gene is inactive in B. subtilis 168. |
| epsD | 99.649 | 3,286,071 | 3,284,932 | Extracellular polysaccharide synthesis. |
| epsE | 100.000 | 3,284,935 | 3,284,093 | Inhibitor of motility and glycosyltransferase required for EPS biosynthesis. |
| epsF | 99.296 | 3,284,100 | 3,282,964 | Similar to glycosyltransferase. |
| epsG | 99.547 | 3,282,960 | 3,281,857 | Extracellular polysaccharide synthesis. |
| epsH | 99.037 | 3,281,838 | 3,280,801 | Undecaprenyl (UnDP) priming UDP-N-acetyl-glucosamine transferase; synthesis of extracellular poly-N-acetylglucosamine. |
| epsI | 98.236 | 3,280,796 | 3,279,720 | Glycosyltransferase; synthesis of extracellular poly-N-acetylglucosamine. |
| epsJ | 98.454 | 3,279,723 | 3,278,689 | UDP-N-acetyl-glucosamine transferase; synthesis of extracellular poly-N-acetylglucosamine. |
| epsK | 99.605 | 3,278,692 | 3,277,175 | Export of extracellular poly-N-acetylglucosamine. |
| epsL | 100.000 | 3,277,178 | 3,276,570 | Similar to UDP-galactose phosphate transferase; extracellular polysaccharide synthesis. |
| epsM | 99.691 | 3,276,573 | 3,275,926 | UDP-2,4,6-trideoxy-2-acetamido-4-amino glucose acetyltransferase; extracellular polysaccharide synthesis. |
| epsN | 99.062 | 3,275,921 | 3,274,749 | UDP-2,6-dideoxy 2-acetamido 4-keto glucose aminotransferase; required for extracellular polysaccharide synthesis. |
| epsO | 99.793 | 3,274,770 | 3,273,805 | Similar to pyruvyltransferase; extracellular polysaccharide synthesis. |
| galE1 | 99.899 | 1,173,111 | 1,172,119 | UDP glucose 4-epimerase. |
| kinA | 77.669 | 1,349,359 | 1,351,179 | Two-component sensor kinase; phosphorylates Spo0F; part of the phosphorelay. |
| motA | 79.044 | 1,317,745 | 1,316,930 | H+-coupled MotA-MotB flagellar stator. |
| motB | 76.305 | 1,316,958 | 1,316,216 | H+-coupled MotA-MotB flagellar stator. |
| remA | 97.778 | 1,565,578 | 1,565,847 | Transcriptional regulator of the extracellular matrix genes; acts in parallel with SinR, AbrB, and DegU. |
| sigD | 87.451 | 1,640,430 | 1,641,194 | RNA polymerase sigma fator SigD. |
| sigH | 87.931 | 123,748 | 124,385 | RNA polymerase sigma factor SigH; not fully active in laboratory strains due to a mutation (V117A). |
| sigW | 83.156 | 200,648 | 201,211 | RNA polymerase ECF-type sigma factor SigW; required for the adaptation to membrane-active agents; activated by alkaline shock and polymyxin B, vancomycin, cephalosporin C, D-cycloserine, and triton X-100. |
| sinl | 79.096 | 2,446,052 | 2,446,225 | Antagonist of SinR; drives SlrR from the SlrR (LOW) to the SlrR (HIGH) state. |
| sinR | 97.321 | 2,446,259 | 2,446,594 | Transcriptional regulator (Xre family) of post-exponential-phase response genes. |
| sipW | 100.000 | 2,448,076 | 2,447,492 | Bifunctional signal peptidase I that control surface-adhered biofilm formation and processes TasA and TapA. |
| spoOA | 99.251 | 2,412,896 | 2,412,096 | Phosphorelay regulator; initiation of sporulation; coordinates DNA replication and initiation of sporulation by binding to sites close to the oriC. |
| srfAA | 78.807 | 356,838 | 358,849 | Surfactin synthetase/competence. |
| srfAB | 79.825 | 1,892,212 | 1,892,099 | Surfactin synthetase/competence. |
| srfAC | 87.099 | 360,085 | 363,881 | Surfactin synthetase/competence. |
| swrA | 83.481 | 3,360,036 | 3,359,700 | Master activator of flagellar biosynthesis; modulator of DegU activity; converts DegU-P from a repressor to an activator of the fla-che operon; enhances sigD transcription; controls the number of flagellar basal bodies; inactive pseudogene in strain 168. |
| swrB | 81.006 | 1,641,222 | 1,641,400 | Control of SigD activity; required for full SigD activity; activates the flagellar type-III secretion export apparatus by the membrane protein FliP. |
| swrC | 79.906 | 693,419 | 696,562 | Similar to acriflavin resistance protein. |
| tasA | 99.491 | 2,447,427 | 2,446,642 | Major component of the biofilm matrix; forms amyloid fibers. |
| yhxB | 99.541 | 924,407 | 926,149 | Alpha-phosphoglucomutase; required for UDP-glucose synthesis; inhibits FtsZ ring assembly (indirect effect due to a defect in UDP-glucose synthesis); possesses secondary phosphoglucosamine mutase activity. |
| yqxM | 99.554 | 2,448,719 | 2,448,048 | TasA anchoring/assembly protein. |