Figure 5.

Neighbor-Joining tree of small subunit rRNA sequences of chiggers collected from North and South American countries. The chigger small subunit rRNA sequences data were downloaded from GenBank only from peer-reviewed journal publications. The Halacaroides antoniazziae (MG751443) was used as an outgroup. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown below the branches. The tree was drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Kimura 2-parameter method and are in the units of the number of base substitutions per site. This analysis involved 10 nucleotide sequences. All positions containing gaps and missing data were eliminated (complete deletion option). There were a total of 403 positions in the final dataset. Evolutionary analyses were conducted in MEGA11.