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. 2022 Oct 23;13(11):1928. doi: 10.3390/genes13111928

Figure 5.

Figure 5

The application to normalization of estimated reference gene. The normalization used stable (HPRT1, RPL4 and TBP) or the least stable (GAPDH) reference gene. Keratin 10 gene was normalized by HPRT1, RPL4, TBP and GAPDH to prove the influence of reference gene stability. Significant (* p < 0.05; *** p < 0.0001) differences in Keratin 10 expression level between normal and post irradiation skin are presented as an asterisk.