Table 1.
Lists of candidate reference genes and their information.
| Information of Primers | Standard Curve Parameters | ||||||
|---|---|---|---|---|---|---|---|
| Gene Name (Symbol) | Sequence | Base Pair | Accession | R2 | M | B | E |
| β−actin (ACTB) | F : GCACTCTTCCAACCTTCTTTCC | 179 | AF021873.2 | 0.993 | −3.489 | 34.252 | 1.02 |
| R : GCTGTGATTTCCTTCTGCATCC | |||||||
| Glyceraldehyde-3−phosphate dehydrogenase (GAPDH) | F : GGAGAAAGCTGCCAAATATGACG | 118 | NM_001003142.2 | 0.991 | −3.436 | 35.514 | 0.98 |
| R : ACTGTTGAAGTCACAGGAGACC | |||||||
| Tyrosine 3−monooxygenase/tryptophan 5−monooxygenase activation protein, zeta polypeptide (YWHAZ) |
F : GTGAAGAGTCATACAAAGACAGCA | 110 | XM_014118550.1 | 0.992 | −3.511 | 37.145 | 1.01 |
| R : CCCTCCTTCTCCTGCTTCAG | |||||||
| β−glucuronidase (GUSB) | F : ATCTGTAGTCATGTGGTCTGTAGC | 149 | AF019759.1 | 0.996 | −3.332 | 33.257 | 0.99 |
| R : GGTCTGCTTCATAGTTGGAATTGG | |||||||
| Hypoxanthine phosphoribosyl transferase 1 (HPRT1) | F : GACTGAAGAGCTACTGTAATGACC | 168 | NM_001003357.2 | 0.996 | −3.412 | 36.915 | 0.98 |
| R : TCTTTGGATTATGCTCCTTGACC | |||||||
| Ribosomal protein 4 (RPL4) | F : AATGAGAAACCGTCGTCGTATCC | 141 | NM_001252409.1 | 0.992 | −3.355 | 39.041 | 1.01 |
| R : GGAGCAAGTTTCAGAATGTTCAGC | |||||||
| Ribosomal protein S5 (RPS5) | F : TGAAGGAGAAGTATGCCAAGTACC | 188 | XM_533568.5 | 0.995 | −3.435 | 39.145 | 0.97 |
| R : GAGCAGATGGATGATCTCGAAGG | |||||||
| TATA box binding protein (TBP) | F : ATCTGGTATCCCTTACGCTTCG | 137 | XM_849432.4 | 0.995 | −3.498 | 36.972 | 1.03 |
| R : GCAAGAGAGTCTGGTTTGTTTCC | |||||||
| Keratin 10 | F : CTCGTGACTACAGCAAATACTACC | 105 | NM_001013425.1 | 0.997 | −3.501 | 33.783 | 1.01 |
| R : TGGCATTGTCGATCTGAAGC | |||||||