Table 2.
Stability of reference gene analyzed by Bestkeeper.
| GAPDH | YWHAZ | TBP | RPS5 | RPL4 | ACTB | HPRT1 | GUSB | |
|---|---|---|---|---|---|---|---|---|
| SD [±CT] | 1.198596 | 0.993889 | 0.95946 | 0.950324 | 0.93821 | 0.935185 | 0.901281 | 0.654506 |
| CV [% CT] | 7.732737 | 5.277597 | 3.791541 | 5.959286 | 3.811451 | 4.742051 | 4.112048 | 2.958517 |
Identification of expression of reference gene stability used Bestkeepr. The most stable reference gene to be placed the right side of table and the most unstable reference gene to be placed the left side. The data present coefficient of variance (CV s.e.m.) and SD (±Ct) values. The reference gene of presenting a s.e.m higher than 1 was shown in bold letter.