FIG. 4.
Primary structures of RepFIIA and the ccdAB regions. (A) Comparison of the RepFIIA region of pB171 and the corresponding regions of the R100 (NR1), F, and pSU233 plasmids. ORFs are indicated by arrows under the DNA of the pB171 plasmid. Nucleotide positions are indicated on the line. Homologous sequences of NR1 (accession no. X12776), F (M12987), and pSU233 (X55893) are indicated by lines under arrows, showing homologous regions in nucleotide position in each sequence with percent identity. Note that only the sequence of pB171 corresponding to the copB region is replaced by pSU233. (B) Schematic representation of the primary structure of the plasmid maintenance region. ORFs are indicated by arrows under the DNA of pB171. The nucleotide sequence of the ccdAB region showed high homology with that of the F plasmid (M12987) and the pO157 plasmid of E. coli O157:H7 (AB011549). The impB region showed high homology with the PT110 plasmid of Salmonella typhimurium (X53528) and with the SA100 plasmid of S. flexneri (AF079316).