Figure 2.

Effect of miRNA mimics transfection on Gab2 protein expression. (A) Expression of miRNA mimics. (B) Effects of four miRNA mimics on Gab2 protein expression, as checked by Western blotting. Tubulin was used as a total protein control. (C,D) Effects of hsa-miR-9 mimic and hsa-miR-181a mimic on Gab2, mRNA expression, as analyzed by RT-PCR. Empty: nontransfected; mi-Ctrl: mimic negative control. The results are presented as the means ± SDs. Statistical comparisons of values were made using Student’s t-test. ns, nonsignificant; ** p < 0.01.