Table 2.

Reagents required for efficient adenovirus genome engineering.

Reagents	Supplier	Specific Handling	Storage Conditions
AbsI	GeneOn		−20 °C
Agarose	Peqlab		RT
Ampicillin sodium salt	Carl Roth	Stock solution in 70% ethanol (−20 °C)	4 °C
Ampuwa	Fresenius Kabi		RT
Chloramphenicol	Carl Roth	Stock solution in 70% ethanol (−20 °C)	RT
DNA ladder 1 kb	Peqlab		−20 °C
E. coli GB05-dir	Gene Bridge	Streak on LB-plate for use	−80 °C
E. coli GBRed-GyrA462	Gene Bridge	Streak on LB-plate for use	−80 °C
E. coli GBdir-pir116-gyrA462	Gene Bridge	Streak on LB-plate for use	−80 °C
Electroporation cuvettes 1 mm (reusable)	Cell projects		RT
Ethanol	Carl Roth		RT
Ethidium bromide	Carl Roth	Toxic, handing with care	4 °C
Gel and PCR cleanup	BioBudget		RT
Glycogen	Carl Roth	Stock solution in ddH2O (−20 °C)	4 °C
I-PpoI	Promega		−20 °C
Isopropanol	Carl Roth	Toxic, handing with care	RT
L-arabinose	Sigma-Aldrich	10% stock solution (−20 °C)	RT
Lysogene Broth (LB) powder	Carl Roth		RT
Nitrocellulose filters (0.025 µm)	Merck-Millipore		RT
Petri dish 10 cm	Sarstedt
Phase Lock Gel™ heavy	VWR		RT
Phenol:chloroform:isoamyl-alcohol (25:24:1)	Carl Roth	Work in fume hood, dispose of all waste as hazardous waste	4 °C
PrimeSTAR® Max DNA Polymerase	TAKARA		−20 °C
pR6K-ccdB-Amp	[7]		−20 °C
pR6K-ccdB-Amp-(IP)	This study		−20 °C
pR6K-(IP)-ccdB-Amp-(IP)	This study		−20 °C
pR6K-(AB)-ccdB-Amp-(AB)	This study		−20 °C
pR6K-hyg-spect-PBs	[19]		−20 °C
Primer (≤50 nucleic acids)	Eurofins Genomics		−20 °C
Primer (>50 nucleic acids)	Sigma-Aldrich	With HPLC purity	−20 °C
Restriction enzymes	NEB		−20 °C
Sodium acetate	Carl Roth		RT
Streptomycin	Carl Roth		RT

RT, room temperature.