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. 2022 Nov 8;13(11):2070. doi: 10.3390/genes13112070

Figure 6.

Figure 6

DNA methylation in the testis and sperm in absence of estrogen synthesis. (A) Immunodetection of 5mC and 5hmC in control and CYP19A1−/− testis (green). Nuclei were stained in blue (DAPI). (B) Percentage of sperm DNA methylation from control and CYP19A1−/− male rabbits determined by Luminometric Methylation Assay (LUMA). The median is shown in red. Mann–Whitney test: nonsignificant.