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. 2022 Nov 7;13(11):2056. doi: 10.3390/genes13112056

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Immunofluorescence analysis of TC1-DSG2 tumor sections. Tumors were harvested when they reached a volume of 500–600 mm³, embedded in OCT, and sectioned. (A) Sections were stained for (human) DSG2 (green) and the epithelial marker claudin 7 (red). Visible are nests of human DSG2-postitive tumor cells embedded in tumor stroma cells (mouse-derived). (B) Sections were stained for the extracellular matrix/stroma protein (red) and the endothelial/blood vessel cell marker CD31 (green). The images show a vascularized tumor with typical stroma components.