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Effect of SPD–FSAP on thrombin- or VEGF-induced permeability. (A) 2 unit/mL thrombin with/without 10 µg/mL recombinant WT–SPD–FSAP or MI–SPD–FSAP were applied to HUVECs for 2 h. (B) 50 ng/mL VEGF with/without 10 µg/mL recombinant WT–SPD–FSAP or MI–SPD–FSAP were applied to HUVECs for 16 h. Permeability ratio was calculated as the rate relative to control. Data are presented as mean ± SEM; n = 3. * p < 0.05 N represents 3 independent experiments, with 3 replicates in each experiment.
