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. 2022 Nov 16;11:e83346. doi: 10.7554/eLife.83346

Figure 4. ssRNA40 and fecal extract do not modify Piezo1 mechanotransduction.

Figure 4.

(A) Example whole-cell voltage-clamp recordings of Piezo1-transfected HEK293 cells during mechanical stimulation. Top traces indicate the magnitude of plasma membrane indentation in 1 µm steps, and bottom traces show whole-cell currents elicited by the stimuli. Vehicle, 10 µg/mL ssRNA40, 5 mg/mL fecal extract, or 30 µM Yoda1 were bath-applied 5 min prior to recording. (B) Piezo1 current versus membrane indentation demonstrates the stimulus–response relationships across conditions. n = 8–19 cells per condition are shown as mean ± SEM. (C) Quantification of mechanically evoked current amplitude, threshold, and inactivation. Individual cell responses are plotted: n = 14 vehicle, n = 8 ssRNA40, n = 19 fecal extract, and n = 10 Yoda1. Error bars represent mean ± 95% CI. One-way ANOVA with Bonferroni correction: n.s. p≥0.05, *p<0.05.