| Strain, strain background (Mus musculus) |
C57BL/6 |
Jax |
Strain # 000664 |
Maintained in Chesler lab |
| Other (rodent chow) |
Dietary extract |
LabDiet |
Prolab RMH 1800 5LL2 |
Autoclaved in Chesler lab and used in Chesler and Patapoutian labs |
| Biological sample (rodent feces) |
Fecal extract |
This study |
Derived from C57BL/6 mice |
Freshly isolated in Chesler lab and used in Chesler and Patapoutian labs |
| Cell line (M. musculus) |
Neuro-2a cells |
ATCC |
CCL-131 |
Maintained in Chesler lab |
| Cell line (M. musculus) |
Piezo1-KO Neuro-2a cells |
Max Delbrück Center for Molecular Medicine; Moroni et al., 2018
|
|
Maintained in Chesler lab |
| Cell line (Homo sapiens) |
HEK293 cells |
ATCC |
CRL-1573 |
Maintained in Chesler lab |
| Cell line (H. sapiens) |
PIEZO1-KO HEK293 cells |
Scripps Research; Dubin et al., 2017
|
|
Maintained in Patapoutian lab |
| Cell line (H. sapiens) |
GCaMP6s HEK293 cells |
This study |
Derived from the Flp-In T-Rex 293 cell line (Thermo Fisher, R78007) |
Maintained in Chesler lab |
| Cell line (Rattus norvegicus) |
RIN14B cells |
ATCC |
CRL-2059 |
Maintained in Chesler lab |
| Recombinant DNA reagent |
CMV-mPiezo1-IRES-eGFP |
Addgene |
80925 |
Used in Chesler lab |
| Recombinant DNA reagent |
CMV-mPiezo1 |
This study |
Derived from pcDNA5-FRT (Thermo Fisher, V601020) |
Used in Chesler lab |
| Recombinant DNA reagent |
CMV-hPIEZO1 |
This study |
Derived in A. Patapoutian lab |
Used in Patapoutian lab |
| Recombinant DNA reagent |
CMV-GCaMP6s |
Addgene |
40753 |
Used in Chesler lab |
| Recombinant DNA reagent |
pLV-CMV-GCaMP6s-PGK-Hyg |
This study |
Derived from pLV-CMV-PGK-Hyg (Cellomics Technology, LVR-1046) |
Used in Chesler lab |
| Chemical compound, drug |
ssRNA40 |
Invivogen |
A40-41-02 |
Used in Chesler and Patapoutian labs |
| Chemical compound, drug |
ssRNA41 |
Invivogen |
A41-41-02 |
Used in Chesler lab |
| Chemical compound, drug |
Yoda1 |
Sigma-Aldrich |
SML558-5MG |
Used in Chesler and Patapoutian labs |
| Chemical compound, drug |
Ionomycin |
Sigma-Aldrich |
I0634 |
Used in Chesler lab |
| Chemical compound, drug |
Gadolinium (III) chloride |
Sigma-Aldrich |
439770 |
Used in Chesler lab |
| Chemical compound, drug |
AITC |
Sigma-Aldrich |
377430 |
Used in Chesler lab |
| Chemical compound, drug |
A-967979 |
Sigma-Aldrich |
SML0085 |
Used in Chesler lab |
| Chemical compound, drug |
Fluo-4 AM dye |
Fisher Scientific |
F14201 |
Used in Chesler lab |
| Chemical compound, drug |
Fluo-8 AM dye |
AAT Bioquest |
21080 |
Used in Patapoutian lab |
