Table 1.
SNP Position | Primer Sequence (5′-3′) | PCR Size (bp) | Ta (°C) |
---|---|---|---|
g.15177692G>A | F:5′-TTGGACCAGGAACAGAGCTG-3′ | 539 | 58 |
R:5′-GTCGCTCAGAACTCACCATA-3′ | |||
g.15178184C>T | F:5′-GACAGCAAAGGGGAGGAAGG-3’ | 317 | 58 |
R:5′-TGCTAGGGTTAGTGTATGAGATTGA-3′ | |||
g.15179224C>T | F:5′-CACTTCATCCTTCGGTTCTGGA-3′ | 507 | 60 |
R: 5′-GCCACTCTGCACTTGTAAACA-3′ |
Note: Ta, annealing temperature.