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. 2022 Nov 21;13(11):2175. doi: 10.3390/genes13112175

Table 1.

Primer sequences used for single nucleotide polymorphisms (SNPs) validation.

SNP Position Primer Sequence (5′-3′) PCR Size (bp) Ta (°C)
g.15177692G>A F:5′-TTGGACCAGGAACAGAGCTG-3′ 539 58
R:5′-GTCGCTCAGAACTCACCATA-3′
g.15178184C>T F:5′-GACAGCAAAGGGGAGGAAGG-3’ 317 58
R:5′-TGCTAGGGTTAGTGTATGAGATTGA-3′
g.15179224C>T F:5′-CACTTCATCCTTCGGTTCTGGA-3′ 507 60
R: 5′-GCCACTCTGCACTTGTAAACA-3′

Note: Ta, annealing temperature.