Figure 2.

Infectious monkeypox virus detected in saliva samples from patients

(A) Representative cytopathic effect in BSC-1 cells inoculated with a fraction of saliva sample from a patient and examined daily for cytopathic effect; images show viral spreading through the cell culture at 0, 3, and 5 days after infection. (B) Box and whisker plot showing the distribution of monkeypox virus loads from saliva samples containing (red) or not containing (blue) viable virus (n=41). (C, D) Identification of monkeypox virus particles (green arrows) in saliva samples by electron microscopy after negative staining; the inset shows the magnification of the indicated virion. (E) Monkeypox virus particle from a pustular lesion identified by electron microscopy after negative staining.