TABLE 2.

Cell types and signaling pathways associated with each mode of single cell migration.

Migration mode	Examples of cell types capable of using each migration mode	Essential signaling pathways and proteins
Pulled nuclear piston	Primary human mesenchymal cells, fibrosarcoma cells	RhoA-ROCK-NMII, Tpm 1.6, plectin, vimentin
Pushed nuclear piston	Mesenchymal stem cells	TRPV4, Piezo1, nesprin 2
Mesenchymal	Primary human mesenchymal cells, single tumor cells	NEDD9, DOCK3, Rac1, Arp2/3, NMII, MMP
Unstable bleb amoeboid	Single tumor cells, T-cells	FilGAP, ARHGAP22, RhoA-ROCK-NMII, JAK1, STAT3, ezrin, moesin
Stable bleb amoeboid (A2)	Single tumor cells, immune cells	Erk, NMII, Eps8, filamin-A, fascin-1, cofilin-1
Rapid amoeboid (A1)	Normal fibroblasts, normal epithelial cells	Not determined
Osmotic engine	Single tumor cells	RhoA, TRPV4, NHE-1, SWELL-1