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. 2022 Oct 24;12(17):7476–7490. doi: 10.7150/thno.75421

Figure 2.

Figure 2

SIAIS361034 significantly and selectively inhibits Hh pathway activity in vitro. (A, B) Dual-luciferase reporter analysis of the inhibitory effect of SIAIS361034 and SIAIS361034NC against the Hh activity in light II cells exposed to SAG (100 nM) with or without various concentrations of SIAIS361034 and SIAIS361034NC as indicated for 36 h. Data represent mean ± SD (n = 2). (C) IC50 values of SIAIS361034 and SIAIS361034NC inhibiting the Gli-luciferase activity provoked by SAG. IC50 values were determined by nonlinear regression dose response fit in Graphpad prism 8. (D) RT-qPCR analysis of the effect of SIAIS361034 on the mRNA expression of Hh pathway target genes Gli1 and Ptch1 in the NIH-3T3 cells exposed to SAG with or without SIAIS361034 for 36 h as indicated. Data represent mean ± SD (n = 3).