Fig. 8.

p62 analysis confirms autophagy impairment in UTD a p62-SQSTM1 representative images and b WB quantification in all the tested cell lines treated with bleomycin for 3 h and then kept in recovery course for 24 h. WT hT cells displayed the same quantity of p62 both at basal and after drug treatment but increased its amount upon recovery condition. TD cells presented a reduced amount of p62 in comparison with UTD at basal condition, despite being significant only compared to TD 3-52. After bleomycin treatment TD 4-53 and TD SINT degraded p62, improving the autophagy flux and supporting LC3B results. In contrast, p62 quantity is not affected by the treatment in TD 3-52 and TD miniATM. After 24 h of recovery, it enhanced in TD 4-53, TD SINT and TD miniATM. In UTD, p62 enhanced after 3 h of drug treatment and decreased after the recovery condition. Red asterisks refer to intra sample comparison, while the black ones show statistical comparison with the control cell line UTD (Friedman test followed by Dunn’s test). Graphs show mean with SEM, n = 7