TABLE 5.
Rabbit antibody response to TT or OMP elicited by conjugates
| Immunogen | Injection no. | GM (range) ELISA unitsa or titersb for:
|
|
|---|---|---|---|
| IgG | IgM | ||
| dLOS-TT | 1 | 1 | 1 (0.3–1) |
| 2 | 81 (27–243) | 6 (0.3–1) | |
| dLOS-TT + adjuvant | 1 | 2 (1–3) | 2 (1–3) |
| 2 | 427 (243–729) | 2 (1–3) | |
| dLOS + TT | 1 | 13 (3–27) | 3 |
| 2 | 246 (81–729) | 16 (9–27) | |
| dLOS-OMP | 1 | 43 (30–90) | 44 (30–90) |
| 2 | 540 (270–810) | 62 (30–90) | |
| dLOS-OMP + adjuvant | 1 | 30 | 30 |
| 2 | 1,260 (810–2,430) | 52 (30–90) | |
| dLOS + OMP | 1 | 269 (90–810) | 52 (30–90) |
| 2 | 1,413 (2,430–7,290) | 52 (30–90) | |
a
Based on a reference serum against TT (720 or 80 U for IgG or IgM) and TT as a coating antigen. Presera were ≤1 U for IgG or IgM.
b
Due to the high OMP background of rabbit presera, titers are expressed a dilution fold higher than those of presera. OMP was used as a coating antigen. GM, geometric mean.