Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Goat polyclonal anti-mCherry | Sicgen | Cat# AB0040, RRID:AB_2333093 |
| Rabbit polyclonal anti-GFP | Thermo Fisher Scientific | Cat# A-11122, RRID:AB_221569 |
| Chicken polyclonal anti-GFP | Aves Labs | Cat# GFP-1020, RRID:AB_10000240 |
| Mouse monoclonal anti-gephyrin | Synaptic Systems | Cat# 147 011C5, RRID:AB_2810214 |
| Mouse monoclonal anti-NeuN | Millipore | Cat# MAB377, RRID:AB_2298772 |
| IB4 (Alexa-647 conjugated) | Molecular Probes | Cat# L21411, RRID:AB_2314665 |
| Donkey anti-Goat IgG Alexa Fluor 546 | Thermo Fisher Scientific | Cat# A-11056, RRID:AB_2534103 |
| Donkey anti-Rabbit IgG Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A-21206, RRID:AB_2535792 |
| Donkey anti-Chicken IgG Alexa Fluor 488 | Jackson ImmunoResearch Labs | Cat# 703-545-155, RRID:AB_2340375 |
| Donkey anti-Mouse IgG Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A-21202, RRID:AB_141607 |
| Donkey anti-Mouse IgG Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A32766, RRID:AB_2762823 |
| Bacterial and virus strains | ||
| AAV9- hSyn-CheRiff-TdT | Boston Children’s viral core | N/A |
| AAV2retro-hSyn-Chronos-GFP | Boston Children’s viral core | N/A |
| RabV-deltaG-GFP | Boston Children’s viral core | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Tamoxifen | Sigma | Cat# T5648–1g |
| Sunflower seed oil | Sigma | Cat# S5007 |
| Urethane | Sigma | Cat# U2500 |
| Isofluorane | Henry Schein | Cat# 029405 |
| NBQX disodium salt | Tocris | Cat# 1044 |
| SR95531 | Tocris | Cat# 1262/10 |
| Strychnine hydrochloride | Sigma | Cat# S8753 |
| Tetrodotoxin citrate | Tocris | Cat# 1069 |
| 4-aminopyridine (4AP) | Tocris | Cat# 0940 |
| Vybrant DiI Cell-Labeling Solution | Thermo Fisher Scientific | Cat# V22885 |
| Paraformaldehyde, reagent grade, crystalline | Millipore Sigma | Cat# P6148–500G |
| Critical commercial assays | ||
| RNAscope Fluorescent Multiplex Assay | ACD Bio | Cat# 320850 |
| RNAscope Protease III & Protease IV Reagents | ACD Bio | Cat# 322340 |
| RNAscope Probe Diluent | ACD Bio | Cat# 300041 |
| Experimental models: Organisms/strains | ||
| Mouse: VgatiresCre | The Jackson Laboratory | RRID:IMSR_JAX:016962 |
| Mouse: RorβiresCre | The Jackson Laboratory | RRID:IMSR_JAX:023526 |
| Mouse: RorβCreER | The Jackson Laboratory | RRID:IMSR_JAX:030290 |
| Mouse: Pvalb2a-Cre | The Jackson Laboratory | RRID:IMSR_JAX:012358 |
| Mouse: Kcnip2-CreER | The Jackson Laboratory | RRID:IMSR_JAX:030385 |
| Mouse: CCKiresCre | The Jackson Laboratory | RRID:IMSR_JAX:012706 |
| Mouse: PKCγCreER | The Jackson Laboratory | RRID:IMSR_JAX:030289 |
| Mouse: Lbx1FlpO | Bourane et al., 2015 | N/A |
| Mouse: Vgat-2A-FlpO | The Jackson Laboratory | RRID:IMSR_JAX:029591 |
| Mouse: Gad2T2A-NLS-mCherry | The Jackson Laboratory | RRID:IMSR_JAX:023140 |
| Mouse: Cdx2-Cre | Coutaud and Pilon, 2013 | N/A |
| Mouse: Cdx2-NSE-FlpO | Abraira et al., 2017 | RRID:IMSR_JAX:030288 |
| Mouse: AdvillinCre | Hasegawa et al., 2007 | RRID:IMSR_JAX:032536 |
| Mouse: AdvillinFlpO | Choi et al., 2020 | N/A |
| Mouse: TrkBCreER | Rutlin et al., 2015 | MGI:5616440 |
| Mouse: RetCreER | Luo et al., 2009 | N/A |
| Mouse: TrkCCreER | Bai et al., 2015 | N/A |
| Mouse: Nav1.8Cre | The Jackson Laboratory | RRID:IMSR_JAX:036564 |
| Mouse: CalcaCreER | Song et al., 2012 | MGI:5460801 |
| Mouse: Calca-FlpE | Choi et al., 2020 | N/A |
| Mouse: MrgprdCre | Rau et al., 2009 | MGI:3852395 |
| Mouse: MrgprdCreER | Olson et al., 2017 | RRID:IMSR_JAX:031286 |
| Mouse: R26LSL-ChR2-YFP | Madisen et al., 2012 | RRID:IMSR_JAX:012569 |
| Mouse: R26LSL-eNpHR3.0-YFP | Madisen et al., 2012 | RRID:IMSR_JAX:014539 |
| Mouse: R26LSL-synaptophysin-tdTomato | Madisen et al., 2012 | RRID:IMSR_JAX:012570 |
| Mouse: TauLSL-mGFP-ires-NLS-lacZ | Hippenmeyer et al., 2005 | RRID:IMSR_JAX:021162 |
| Mouse: Vgatflox | The Jackson Laboratory | RRID:IMSR_JAX:012897 |
| Mouse: Gabrb3flox | The Jackson Laboratory | RRID:IMSR_JAX:008310 |
| Mouse: TrkBflox | Liu et al., 2012 | RRID:IMSR_JAX:022362 |
| Mouse: Atoh1flox | Shroyer et al., 2007 | RRID:IMSR_JAX:008681 |
| Mouse: Piezo2flox | Woo et al., 2014 | RRID:IMSR_JAX:027720 |
| Mouse: RC::PFtox | Kim et al., 2009 | N/A |
| Oligonucleotides | ||
| Mm-Piezo2-E43-E45-C3 | ACD Bio | Cat# 439971-C3 |
| Mm-Scn10a-C2 | ACD Bio | Cat# 426011-C2 |
| Mm-Calca-tv2tv3 | ACD Bio | Cat# 420361 |
| Mm-Mrgprd | ACD Bio | Cat# 417921 |
| Mm-Nefh | ACD Bio | Cat#: 443671-C2 |
| Mm-Rorβ | ACD Bio | Cat# 444271-C2 |
| Mm-Slc32a1 | ACD Bio | Cat# 319191 |
| Software and algorithms | ||
| JRCLUST | Jun et al., 2017 | https://github.com/JaneliaSciComp/JRCLUST |
| MATLAB | Mathworks | https://www.mathworks.com/products/matlab.html; RRID: SCR_001622 |
| Python | Van Rossum and Drake, 1995 | https://www.python.org/ |
| Clampex 10 | Molecular Devices | https://www.moleculardevices.com; RRID: SCR_011323 |
| ImageJ | Schneider et al., 2012 | https://imagej.nih.gov/ij/ |
| Other | ||
| Galvo mirrors | Cambridge Technologies | 6210H |
| F-theta lens | Thorlabs | FTH100-1064 |
| Multielectrode arrays | Cambridge Neurotech | ASSY-37 H4 |
| Multielectrode arrays | Neuronexus | A1×32-Poly3-5mm-25s-177-A32 and A1×32-Poly2-5mm-50s-177-OA32 |
| RHD USB interface board Intan Technologies C3100 | RHD USB interface board Intan Technologies C3100 | RHD USB interface board Intan Technologies C3100 |
| Patch-clamp amplifier | Molecular Devices | Multiclamp 700A |
| Acquisition system | Molecular Devices | Digidata 1440A |