KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-GABA | Sigma | Cat# A2052 RRID: AB_477652 |
| Mouse monoclonal anti-ChAT | Developmental Studies Hybridoma Bank | Cat# 4B1 RRID: AB_528122 |
| Rabbit polyclonal anti-vGluT | Daniels et al.33 | RRID: AB_2567386, |
| Chicken polyclonal anti-GFP | Invitrogen | Cat# A10262 RRID: AB_2534023 |
| Rabbit polyclonal anti-dsRed | Clontech | Cat # 632496 RRID: AB_10013483 |
| Mouse monoclonal anti-BRP | Developmental Studies Hybridoma Bank | Cat# nc82 RRID: AB_2314866 |
| Alexa Fluor 488 Goat anti-Chicken IgY | Invitrogen | Cat# A11039 RRID: AB_2534096 |
| Alexa Fluor 568 Goat anti-Mouse IgG | Invitrogen | Cat# A11031 RRID: AB_144696 |
| Alexa Fluor 647 Goat anti-Mouse IgG | Invitrogen | Cat# A21235 RRID: AB_2535804 |
| Alexa Fluor 568 Goat anti-Rabbit IgG | Invitrogen | Cat# A11011 RRID: AB_143157 |
| Rabbit polyclonal anti-GFP | Thermo Fisher | Cat# A11122 RRID: AB_221569 |
| Alexa Fluor 488 Goat anti-Rabbit IgG | Thermo Fisher | Cat# A27034 RRID: AB_2536097 |
| Alexa Fluor 568-conjugated streptavidin | Thermo Fisher | Cat# S11226 |
| Bacterial and virus strains | ||
| N/A | ||
| Biological samples | ||
| N/A | ||
| Chemicals, peptides, and recombinant proteins | ||
| Collagenase | Sigma | Cat# C5138 |
| Protease XIV | Sigma | Cat# P5147 |
| Dispase | Sigma | Cat# D4693 |
| Escin | Santa Cruz Biotechnology | Cat# 6805-41-0 |
| Mecamylamine | Sigma | Cat# M9020 |
| Critical commercial assays | ||
| N/A | ||
| Deposited data | ||
| N/A | ||
| Experimental models: Cell lines | ||
| N/A | ||
| Experimental models: Organisms/strains | ||
| iso31 | Bloomington Drosophila Stock Center | BDSC# 5905 |
| R19G02-GAL4 | Bloomington Drosophila Stock Center | BDSC# 48860 |
| SS50574 (VT017491-AD, VT043927-DBD) | Janelia | SS50574 |
| R60D05-GAL4 | Bloomington Drosophila Stock Center | BDSC# 39247 |
| R15C03-GAL4 | Bloomington Drosophila Stock Center | BDSC# 47865 |
| R58H05-GAL4 | Bloomington Drosophila Stock Center | BDSC# 39198 |
| R46C03-GAL4 | Bloomington Drosophila Stock Center | BDSC# 50258 |
| R30G03-GAL4 | Bloomington Drosophila Stock Center | BDSC# 49646 |
| R69F08-GAL4 | Bloomington Drosophila Stock Center | BDSC# 39499 |
| R58H05-QF2 #7 | Blum et al.2 | |
| R58H05-QF2 #A | Wu Lab | |
| R58H05-AD (86Fb), R46C03-DBD (vk27) | Blum et al.2 | |
| R30G03-AD (86Fb), R58H05-DBD (vk27) | Liu et al.1 | |
| R19G02-LexA | Bloomington Drosophila Stock Center | BDSC# 52550 |
| UAS-ChAT-miR1-A | Wu Lab | |
| UAS-mCD8-GFP | Bloomington Drosophila Stock Center | BDSC# 5137 |
| UAS-dTRPA1 | Bloomington Drosophila Stock Center | BDSC# 26263 |
| UAS-TNT | Bloomington Drosophila Stock Center | BDSC# 28838 |
| UAS-CD4-tdGFP | Bloomington Drosophila Stock Center | BDSC# 35836 |
| UAS-mtdTomato | Bloomington Drosophila Stock Center | BDSC# 30124 |
| ChaTMI04508-GAL4 | Bloomington Drosophila Stock Center Diao et al.17 | BDSC# 60317 |
| vGluTMI04979-GAL4 | Bloomington Drosophila Stock Center. Diao et al.17 | BDSC# 60312 |
| UAS-Stinger | Bloomington Drosophila Stock Center | BDSC# 84277 |
| QUAS-dTRPA1-7 | Gift of C. Potter | |
| UAS-spGFP1-10 | Bloomington Drosophila Stock Center | BDSC# 93016 |
| lexAop-CD4-spGFP11 | Bloomington Drosophila Stock Center | BDSC# 93019 |
| LexAop-tdTomato | Bloomington Drosophila Stock Center | BDSC# 77138 |
| UAS-syt-GFP, UAS-DenMark | Bloomington Drosophila Stock Center | BDSC# 33065 |
| 10XUAS-syn21-GFP-p10 (attP2) pJFRC81 | Gift of G. Rubin | Pfeiffer et al.34 |
| 20XUAS-IVS-dTRPA1 (attP18) pJFRC124 | Gift of G. Rubin | |
| ppk-Gal80 | Gift of W. Joiner | |
| MB247-GAL4 | Bloomington Drosophila Stock Center | BDSC# 50742 |
| 20XUAS-6XGFP | Bloomington Drosophila Stock Center | BDSC# 52261 |
| 10XQUAS-6XGFP | Gift of C. Potter | |
| Oligonucleotides | ||
| Oligo 5’
ATAGAATTCCGCCAGATCTTTTAAAGTCCACAACTCATCAAGGAAAAT GAAAGTCAAAGTTGGCAGCTTACTTAAACTTAATCACAGCCTTTAAT GTACCGCTGGCTGGGAACTTTCATTAAGTTAATATACCATATCTAATTAAAGT TCCCAGCCAGCGGTGTACCTAAAGTGCCTAACATCATTATTTAATTTTTTTTTTTTT TGGCACACGAATAACCATGCCGTTTTGGATCTTTTAAAGTCCACAACTCATCAAGGAAAATGAAAGTCAAAGTTGGCAGCTTAC TTAAACTTAATCACAGCCTTTAATGTAGATGCACGAGCTGTTCAAC GATAAGTTAATATACCATATCTATCTTTGAACAGCTCGTGCATCTGTACCTAAA GTGCCTAACATCATTATTTAATTTTTTTTTTTTTTGGCACACGAATAACCATGCCGTTTTGGATCCGTGCGGCCGCAAC 3’ |
GeneArt | UAS-ChaT-miR |
| Recombinant DNA | ||
| pUAST | Brand and Perrimon35 | |
| Software and algorithms | ||
| FIJI | Open Source |
https://imagej.net/software/fiji/ RRID: SCR_002285 |
| MATLAB | Mathworks | RRID: SCR_001622 |
| Custom MATLAB Scripts for sleep analysis | Blum et al.2 | https://github.com/marknwulab/Blum-et-al.-2020 |
| Custom Python code in Jupyter Notebook | Wu Lab | https://github.com/margiezilla/Ho_etal_2022 |
| Prism 7 | Graphpad | RRID: SCR_002798 |
| DAMFileScan311 | Trikinetics | N/A |
| DAMFileScan113 | Trikinetics | N/A |
| Zen Black | Zeiss | RRID:SCR_013672 |
| Jupyter Notebook | https://jupyter.org/ | RRID:SCR_018315 |
| Python Programming Language | http://www.python.org/ | RRID:SCR_008394 |
| Other | ||
| Dental Wax | GC Corp | Cat# 27B2X00008000016 |
| DAM2 Activity Monitors | Trikinetics | Cat# DAM2 |
| LC-4 Light Controller | Trikinetics | Cat# LC4 |
| PSIU9 Power Supply Interface | Trikinetics | Cat# PSIU9 |
| Vortexer with Vortex Mounting Plate | Trikinetics | Cat# TVOR-120 |
| Laser-cut Adaptor Plate for Vortexer | Wu Lab | https://github.com/marknwulab/Blum-et-al.-2020 |
| DVX-2500 Digital Multi-tube Vortexer | VWR | DVX-2500 |