KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-Arp2 | Abcam | Cat#ab49674; RRID:AB_867730 |
| goat anti-mouse Alexa Fluor 647 | Thermo Fisher | Cat#A21236; RRID:AB_2535805 |
| rabbit anti-HA-Tag | Cell Signaling Technology | Cat#C29F4; RRID:AB_1549585 |
| mouse anti-RNAP | BioLegend | Cat#8RB13; RRID:AB_2566583 |
| Bacterial and virus strains | ||
| Mycobacterium marinum M strain/pMSP12:cerulean | Oehlers et al.97 | N/A |
| Mycobacterium marinum M strain/pMSP12:tomato | Cambier et al.98 | N/A |
| Mycobacterium marinum ΔesxM | This paper | N/A |
| Mycobacterium marinum ΔesxM + hsp60:esxM | This paper | N/A |
| Mycobacterium tuberculosis 6020 auxotroph | Sambandamurthy et al.66 | N/A |
| Mycobacterium tuberculosis 6020 + msp 12::cerulean | This paper | N/A |
| Mycobacterium tuberculosis 6020 + msp 12::cerulean-hsp60:esxM | This paper | N/A |
| Mycobacterium tuberculosis H37Rv | BEI Resources, NIAID | NR-13648 |
| Mycobacterium tuberculosis NCG | This paper | N/A |
| Mycobacterium tuberculosis H37Rv/msp12:cerulean | This paper | N/A |
| Mycobacterium tuberculosis NCG/msp12:cerulean | This paper | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| 7H10 | Difco | Cat#262710 |
| Trizol | Ambion | Cat#15596026 |
| Sodium chloride | Fisher Scientific | Cat#S271 |
| Potassium chloride | VWR | Cat#BDH9258 |
| Calcium chloride | VWR | Cat#BDH9224 |
| Magnesium chloride | Ward’s Scientific | Cat#470301 |
| 1-phenyl-2-thiourea | Sigma-Aldrich | Cat#P7629 |
| Tricaine-S (MS-222) | Syndel | ANADA#200–226 |
| Low melting point agarose | Fisher Scientific | Cat#BP165–25 |
| CK-666 Arp2/3 inhibitor | Sigma-Aldrich | Cat#182515 |
| CK-689 inactive control | Sigma-Aldrich | Cat#182517 |
| Dimethyl sulfoxide (DMSO) | Fisher Scientific | Cat#BP231 |
| BP Clonase II | Thermo Fisher | Cat#11789020 |
| HyClone HyQTase Cell Detachment Reagent | Fisher Scientific | Cat#SV3003001 |
| DAPI Fluoromount-G | SouthernBiotech | Cat#0100–20 |
| RPMI-1640 | Sigma-Aldrich | Cat#R8758 |
| GlutaMAX Supplement | Thermo Fisher | Cat#35050061 |
| Phorbol-12-myristate-13-acetate (PMA) | Sigma-Aldrich #R8758 | Cat#P148 |
| Sodium pyruvate | Gibco | Cat#11360 |
| Recombinant human IL-3 | Peprotech | Cat#200–03 |
| Recombinant human M-CSF | Peprotech | Cat#300–25 |
| β-Estradiol | Sigma-Aldrich | Cat#E2758 |
| Alexa Fluor 555 phalloidin stain | Thermo Fisher | Cat#A34055 |
| Hygromycin B solution | Invitrogen | Cat#10687010 |
| 7H9 | Difco | Cat#271310 |
| OADC | Sigma-Aldrich | Cat#M0678 |
| Neonate-80 | Fisher Scientific | Cat#BP337 |
| TRIzol Reagent | Invitrogen | Cat#15596026 |
| RNase-free DNase I | New England BioLabs | Cat#M0303S |
| Luna universal qPCR master mix | New England BioLabs | Cat#M3003X |
| Tyloxapol | Sigma-Aldrich | Cat#T8761 |
| cOmplete EDTA-free protease inhibitor cocktail | Millapore Sigma | Cat#11836170001 |
| Super-Signal West Pico PLUS | Thermo Fisher | Cat#34580 |
| Super-Signal West Femto Maximum Sensitivity | Thermo Fisher | Cat#34095 |
| Prolong Gold Anti-fade mounting solution | Invitrogen | Cat#P36934 |
| Critical commercial assays | ||
| mMessage mMachine T7 Kit | Thermo Fisher | Cat#AM1344 |
| RNeasy Plus Mini Kit | Qiagen | Cat#74134 |
| Zymo Direct-zol RNA Miniprep Kit | Thomas Scientific | Cat#1159U94 |
| DC Protein Assay Kit | Bio-Rad | Cat#5000111 |
| Pierce Micro BCA Protein Assay Kit | Thermo Fisher | Cat#23235 |
| Deposited data | ||
| Mtb genomic sequencing | NCBI BioProject | PRJNA540867 |
| Mtb H37Rv and NCG RNA sequencing | NCBI BioProject | PRJNA872173 |
| Raw mass spectrometry files | MassIVE repository | MSV000090143 |
| Processed mass spectrometry files | PRIDE repository | PDX036131 |
| Experimental models: Cell lines | ||
| BLaER1 | Rappino et al.67 | N/A |
| RAW 264.7 | ATCC | ATCC product TIB-71 |
| THP-1 | ATCC | ATCC product TIB-202 |
| Experimental models: Organisms/strains | ||
| Zebrafish (Danio rerio), *AB wildtype strain | ZIRC | ZDB-GENO-960809–7 |
| Zebrafish, Tg(mfap4:tomato-caax)xt6 | Walton et al.99 | N/A |
| Zebrafish, Tg(mfap4:esxM-p2a-tdTomato) xt49 | This paper | N/A |
| Zebrafish, Tg(mfap4:esxM_Q59X-p2a-tdTomato) xt50 | This paper | N/A |
| Zebrafish, Tg(mfap4:esxB-p2a- mNeonGreen) xt51 | This paper | N/A |
| Zebrafish, Tg(Ola.Sp7:mCherry-Eco.NfsBp46 | Singh et al.100 | N/A |
| Zebrafish, Tg(acp5a:mNeonGreen-CAAX)xt52 | This paper | N/A |
| Mouse, C57Bl/6J | Jackson Laboratories | RRID:IMSR_JAX:000,664 |
| Recombinant DNA | ||
| Oligonucleotides | This paper | Available in Table S5 |
| Software and algorithms | ||
| RNA seq analysis original code | This paper | https://doi.org/10.5281/zenodo.6981721 |
| Phylogenetic analysis original code | This paper | https://github.com/vertgenlab/gonomics |
| BWA | Li and Durbin101 | N/A |
| ClustalW2 | Larkin et al.102 | N/A |
| FIJI/ImageJ2, 2.5.0 | Rueden et al.103; Schindelin et al.104 | N/A |
| iTol | Letunic and Bork105 | N/A |
| Kallisto v0.48 | Bray et al.106 | N/A |
| MtrackJ, 1.5.1 | Meijering et al.107 | N/A |
| RStudio | RStudio Team (2022). RStudio: Integrated Development Environment for R. | N/A |
| RAxML | Stamatakis108 | N/A |
| SAMtools | Li et al.109 | N/A |
| Sleuth v0.30.0 | Pimentel et al.110 | N/A |
| VarScan | Koboldt et al.111 | N/A |