Figure 1.

Specificity characterization of anti-FMRP antibodies (CST7104, Ab17722, and PA5) in the mouse cochlea. (a) Western blot of CST7104 on cochlea samples collected from wildtype (WT) and Fmr1 knockout (KO) mice. (b–c) Immunostaining of CST7104 on WT (b) and Fmr1 KO (c) midmodiolar cross sections. Arrows in (c) point to nonspecific staining along the edge of the modiolous in the Fmr1 KO. (d) Western blot of Ab17722 on cochlea samples collected from WT and Fmr1 KO mice. The asterisk indicates a nonspecific band that was present in both genotypes. (e–f) Immunostaining of Ab17722 on WT (e) and Fmr1 KO (f) cochlea sections. (g) Western blot of PA5 on cochlea samples collected from WT and Fmr1 KO mice. The asterisk indicates a nonspecific band that was present in both genotypes. (h–i) Immunostaining of PA5 on WT (h) and Fmr1 KO (i) cochlea sections. For western blot, each lane was an individual animal. Thirty micrograms of protein were loaded to each lane, with GAPDH or β-tubulin as the loading control. For immunocytochemistry, WT and KO sections were stained and imaged using the same parameters for comparison. Dashed circles outline the SG in the Fmr1 KO. SG, spiral ganglion; TM, tectorial membrane. Scale bar in i = 200 μm, applies to b, c, e, f, h, i.