Fig. 4.
CD147 knockdown inhibits activation of lung fibroblasts upon TGF-β stimulation. a MRC-5 cells were transfected with shRNA for CD147 gene silence and stimulated with TGF-β (10 ng/mL) for 24 h. The gene expression levels of CD147, α-SMA and COL1A1 were detected by real-time PCR. n = 3 samples for each group, one-way ANOVA followed by multiple comparisons. b The statistically significant profile by trend analysis of differentially expressed genes in MRC-5 cells transfected with CD147 shRNA with or without stimulation of TGF-β. c KEGG enrichment analysis of pathways enriched in the profile in b. d Heatmap of significantly enriched genes in the profile. Columns represent samples and rows represent genes. Gene expression levels in the heat maps are z score–normalized values determined by log2[CPM] values