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. 2022 Nov 24;12:20260. doi: 10.1038/s41598-022-24401-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Study design overview. Timeline of the experimental design indicating that the THC-exposed animals underwent ~ 4 months of THC induction preconception with incremental dosing increase until reaching a dose of 2.5 g/7 kg/day (approximately a heavy medical cannabis dose) that was then maintained throughout pregnancy. The age-matched control group were administered a daily edible without THC in addition to their daily chow. Ultrasound was performed at (G60, term is ~ 168 days), and both US and MRI were performed at G85, G110 and G135, and G155 with immediate cesarean delivery following imaging at G155.