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. 2022 Nov 11;13:1035532. doi: 10.3389/fimmu.2022.1035532

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Copyright © 2022 Hanrath, Hatton, Gothe, Browne, Vowles, Leary, Cockell, Cowley, James, Hambleton and Duncan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IFN-I mediates paracrine protection of iPS-macrophages. (A) Immunofluorescence analysis of ZIKV ENV and IFITM3 expression in IFNAR2 ^PT (clone 11) and IFNAR2 ^WT (WT2) iPS-Mϕ (24 h.p.i. ZIKV^FP MOI = 10.0). Representative images from one of three independent experiments are shown. Scale bar = 200 μm. (B) CellProfiler quantification of images in (A) showing proportion of cells expressing ZIKV ENV (left panel) or the ISG IFITM3 (right panel). Mean ± SD of n = 3 independent experiments, ANOVA with Sidak’s test for multiple comparisons. (C) CellProfiler analysis of single cell expression of ENV and IFITM3 in IFNAR2 ^WT and IFNAR2 ^PT iPS-Mϕ from images in (A), n = 2,434 (WT) cells and n = 2,202 (PT) cells respectively. Red dotted lines represent gating. Representative data from one of three independent experiments.