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. 2022 Nov 11;13:1035532. doi: 10.3389/fimmu.2022.1035532

Figure 6.

Figure 6

JAK inhibition recapitulates ZIKV cytopathicity in wild-type iPS-macrophages. (A) Immunofluorescence analysis of cell viability showing representative images of cell death at 72 h.p.i. in IFNAR2 WT (WT1) and IFNAR2 PT (clone 11) iPS-Mϕ treated with RUX (10 uM) or DMSO control (ZIKVFP MOI = 1.0, representative images of n = 4 independent experiments). Scale bar = 200 μm. PI = propidium iodide. (B) CellProfiler quantification of cell viability assay in IFNAR2 PT (clone 11) and IFNAR2 WT (WT1 and WT2) iPS-Mϕ treated with RUX (10 uM) or DMSO control (72 h.p.i. ZIKVFP MOI = 1.0, n = 4 independent experiments and ZIKVMP MOI = 1.0, n = 3 independent experiments). Mean ± SD, ANOVA with Sidak’s test for multiple comparisons. .