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. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: Thorax. 2022 May 25;78(2):151–159. doi: 10.1136/thoraxjnl-2021-218047

Figure 4.

Figure 4

Trauma followed by development of SIRS is associated with circulating dysfunctional CD8+ T cells in human patients. Peripheral blood-derived mononuclear cells were obtained from control patients undergoing elective surgery and from trauma patients on day 0/1 and day 2/3 post injury (n=10 per group). (B) Frequency of CD39+CD73− within blood-derived CD8+ lymphocytes and (C) correlation analysis between mitochondrial DNA and frequency of CD39+CD8+ T cells are shown. (D) Frequency of granzyme B+ cells within CD8+ T cells is displayed. (E) Frequency and representative flow cytometry plots of PD-1+Tim-3+ cells within the CD8+CD39+ subpopulation are also included. (F) Trauma patients were further analysed based on the absence (non-SIRS, n=11) or presence of systemic inflammatory response syndrome (SIRS, n=9). (G–I) Frequencies of CD39+ (G), PD-1+Tim-3+ (H) and granzyme B+ cells (I) within the CD8+ cell population are shown. Data represent mean±SEM p value obtained using Kruskal-Wallis tests followed by Dunn’s multiple comparisons. *p<0.05; **p<0.01; ***p<0.001. mtDNA, mitochondrial DNA; PD-1, programmed cell death 1.