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. 2022 Oct 20;298(12):102630. doi: 10.1016/j.jbc.2022.102630

Figure 3.

Figure 3

Tumorigenic capacity of breast CSCs is enhanced by HOTAIR overexpression.A, HOTAIR overexpressing breast tumor cells were established. Data are shown as means ± SD. B, ALDH1+ (CSC) subpopulations were detected in HOTAIR overexpressed cells by FACS analysis. Results are shown as means ± SD. C, HOTAIR overexpression causes an increased oncosphere-forming capacity in breast cancer stem cells. Cells were magnified by 100 folds and the scale bar represented 10 μm. D, different numbers of HOTAIR-overexpressing or control breast CSC of MCF7 were diluted and subcutaneously implanted into NOD/SCID nude mice. Tumor-free mice were measured 1 month after injection. N = 10 for each group. E, different numbers of HOTAIR-overexpressing or control breast CSC of MDA-MB-453 were diluted and subcutaneously implanted into NOD/SCID nude mice. Tumor-free mice were measured 1 month after injection. N = 10 for each group. F, HOTAIR overexpressing or control breast CSC of MCF7 were diluted and subcutaneously implanted into NOD/SCID nude mice. Tumors were observed over 1 month. N = 10 for each group. G, HOTAIR overexpressing or control breast CSC of MDA-MB-453 were diluted and subcutaneously implanted into NOD/SCID nude mice. Tumors were observed over 1 month. N = 10 for each group. H, overexpression of HOTAIR resulted in elevated expression of pluripotent factors in breast cells as assessed by quantitative real-time PCR. Data are shown as means ± SD. I, the expression of pluripotent factors were determined by Western blot after HOTAIR overexpression. β-actin was used as a control. CSC, cancer stem cell; HOTAIR, Hox transcript antisense intergenic RNA (∗ indicates p < 0.05; ∗∗ indicates p < 0.01.).